Genes identified by RNA-seq as induced during iron deficiency also displayed an enrichment of the activating histone marks, H3K4me3 and H3K27ac at transcription start sites (TSSs) in the low iron condition (e.g. Cdkn1a; Supplementary Fig. 1e, f) when analysed by ChIPmentation. Here, CDKN1A is linked to nutritional disorder.