To further advance knowledge on the extent of cellular dysfunction across different mutation groups in different PD relevant neuron types, we have established an unbiased high-content imaging platform with orthogonal probes to assess lysosomal dysfunction along with alpha-synuclein and tau protein deposition in iPSC derived cortical and ventral midbrain dopamine neurons compared to controls (n = 3-4) without PD or PD gene mutations (n = 3 cell lines per genotype). This evidence concerns the gene MAPT and Parkinson disease.