TP53 and neoplasm: We combined Cre-dependent immunocompetent mouse models (ChAT-IRES-Cre) with an in utero electroporation-induced genetic mouse model of H3K27M-DMG that leverages dual recombinase-mediated cassette exchange to express mutations in H3f3a, Tp53, and Pdgfra in neural precursor cells (MADR).43 After generating tumor cells via in utero electroporation, DMG cells were cultured and subsequently allografted into immunocompetent mice.