OTX2 and Parkinson disease: Midbrain dopaminergic neurons (mDANs), which are affected in Parkinson’s disease, are developed from midbrain floorplate progenitor cells (Kirkeby et al., 2012) that are patterned by exposure to WNT and SHH signaling and express a combination of midbrain markers, such as OTX2 and LMX1A and the floorplate marker FOXA2. We therefore applied WNT and SHH patterning similar to that described by Kirkeby et al. (2012) and Xu et al. (2022) during BMWi patterning in 3 independent hiPSC lines followed by NGN2 overexpression and maturation (Figure 6A).