Our findings revealed a significant upregulation in the mRNA and protein expression levels of ACSL4, TRF, and STEAP3 in the pancreatic tissue of T2DM mice compared to the CON group, alongside a significant downregulation in the mRNA and protein expression of FTL, indicating iron accumulation and depletion in the pancreas. Here, ACSL4 is linked to type 2 diabetes mellitus.