EDN1 and vascular disorder: ANGPTL2 also caused significant upregulation in mRNA levels of factors involved in SSc vasculopathy, such as Edn1 (Figure 5B) and Pdgfb (Figure 5C), in skin explants and the mouse endothelial cell line SVEC4-10, an endothelial cell line derived by SV40 transformation of endothelial cells from mouse axillary lymph node vessels.