Our specific objectives were as follows: (1) demonstrateand quantify tuberosin production from elicited kudzu leaves, (2)assess estrogenic and antiestrogenic effects of tuberosin in MCF-7and T-47D breast cancer cell lines, (3) evaluate receptor-specificactivity for ERα and ERβ using HEK293 cells, (4) determineERα binding affinity for tuberosin compared to E2, and (5) examinethe effects of tuberosin treatment on ER-mediated biological processes,including gene expression (RNA sequencing and PCR), cell proliferation(Alamar Blue), and ERα protein levels (JESS analysis). Here, ESR1 is linked to breast cancer.