Importantly, in a prior study, pMLKL staining was negative in the glomeruli of DKD patients.[18] In this study, expression of RIPK3 and pRIPK3 was increased, while there were no significant changes to expression of MLKL and pMLKL in cultured podocytes treated with HG in vitro. The gene discussed is MLKL; the disease is diabetic kidney disease.