GSDMD and infection: To comprehensively investigate the physiological role of GSDMD‐CT in host defense against viral infection in vivo, we overexpressed GSDMD‐CT in GSDMD‐KO mice via tail vein injection of a plasmid encoding Flag‐tagged mouse GSDMD‐CT with an in vivo transfection reagent for 24 h, followed by EMCV infection through intraperitoneal injection (Figure7a).