To comprehensively investigate the physiological role of GSDMD‐CT in host defense against viral infection in vivo, we overexpressed GSDMD‐CT in GSDMD‐KO mice via tail vein injection of a plasmid encoding Flag‐tagged mouse GSDMD‐CT with an in vivo transfection reagent for 24 h, followed by EMCV infection through intraperitoneal injection (Figure7a). This evidence concerns the gene GSDMD and viral infectious disease.