This separation is driven by CD80, CD83, CD40, and CD206 expression in the direction of tri-culture with untreated A375s (Figure 5E), further supporting the claim that HMDMs cultured with the untreated A375 population assume a complex tumor-associated macrophage state while those cultured with the injured A375 population may be better equipped for antigen presentation. This evidence concerns the gene CD83 and neoplasm.