S100A4 and neoplasm: S100A4 has been identified in numerous studies as a reliable marker for CAFs[30, 31, 32], with expression observed in fibroblasts during post‐embryonic development up to day 8.5.[33, 34] To closely mimic the pathogenesis and tumor microenvironment of human CRC, we established a tumor model in VTN conditional knockout (CKO) mice using the AOM/DSS chemical induction method (Figure 3B).