Using the same kdGJB5-sh2 lentiviral construct (see Fig. 4), we observed consistent results in these cancer cell types: silencing GJB5 expression resulted in mitochondrial depolarization, as indicated by a significant increase in the green monomer form of JC-1 (Fig. 5G), and increased apoptosis, as confirmed by a significant increase in the number of TUNEL-positive cells (Fig. 5H). Here, GJB5 is linked to cancer.