CR2 and kidney disorder: Overall, this manual gating strategy, applied to the total dataset of live cells (25 × 106 to 30 × 106), reproduced the key patterns of cellular features associated with the LN groups (Supplemental Figure 9B), with renal disease activity (Figure 6E) and longitudinal changes, including the decrease in proliferative T-B cells and the increase in CD21 expression in non-proliferative B cells over time, supporting that these populations can readily be identified (Figure 6F).