To explore the potential roles of LUBAC and OTULIN in virus infection, several RNA viruses including vesicular stomatitis virus (VSV) and Sendai virus (SeV), as well as DNA viruses including vaccinia virus (VACV) and herpes simplex virus type 1 (HSV‐1), were used to infect human monocytic leukemia THP‐1 cells, followed by the detection of transcript levels of Hoip, Hoil‐1l, Sharpin, Otulin. The gene discussed is RNF31; the disease is viral infectious disease.