Our study observed an activation of the COX-2/mPGES-1/PGE2 pathway in rats with PD, characterized by increased levels of COX-2, mPGES-1, PGF2α and PGF2α/PGE2 ratio, as well as reduced levels of β-EP and PGE2.Our findings suggest that both low-frequency and high-frequency EA treatments for PD may alleviate uterine spasmodic contractions and improve uterine ischemia and hypoxia by reducing COX-2 and mPGES-1 levels in the serum, uterus, hypothalamus, and spinal cord. The gene discussed is PTGS2; the disease is ischemia.