The interaction between the LGALS9 ligand from DC2 cells and its CD28+PD-1+ Tc cell receptor was higher in HCC (Supplementary Figure S6A), whereas that between the MIF from Mac3 and its CD28+PD-1+ Tc cell receptor was lower in HCC (Supplementary Figure S6B). Here, PDCD1 is linked to hepatocellular carcinoma.