Accordingly, in subcutaneous implantation nude mice models, the mice injected with ECA109‐PGK1 overexpression cells were found to have higher tumour volume and weight and Ki‐67 IHC score, whereas opposite effects were observed in mice injected with PGK1 knockdown cells, indicating that PGK1 promotes tumour tumourigenicity (Figures 2D–F and S2D and E). Here, PGK1 is linked to neoplasm.