PGK1 levels was significantly higher in high HIF‐1α expression group in GSE161533, GSE44021, TCGA‐ESCA and TCGA‐ESCC (Figures S5F–H and S5J), and PGK1 expression had positive correlations with HIF‐1α (Figures S5K–N and 5E). The gene discussed is PGK1; the disease is esophageal squamous cell carcinoma.