The results showed that PGK1 knockdown prolonged the GSK3β protein half‐life, and reduced MYH9‐mediated GSK3β ubiquitination (Figure 4O and P) Western blot analysis indicated that silencing MYH9 abrogated the upregulated GSK3β, phosphorated‐GSK3β and nucleus β‐catenin expression by PGK1 in ESCC cells (Figure 4Q). The gene discussed is MYH9; the disease is esophageal squamous cell carcinoma.