To elucidate the role of SEMA3G in GBM growth, EC-specific Sema3G knockout mice (Cdh5-Cre; Sema3Gf/f) (Supplementary Fig. 3A, B) were used to establish an orthotopic tumor model with syngeneic GL261-Luc cells (Fig. 2D). The gene discussed is CDH5; the disease is neoplasm.