After checking the successful loading of the tumor aggregates of MDA-MB-231 cells into the chip through the “tumor inlet” (Fig. 4B), the different treatments (scDb-hERG1-β1-TRAIL, scDb-hERG1-β1, s-trimer TRAIL, and E4031) were added through an approach named “linear withdraw” (Supplementary Fig. S4B), and their effects on cell vitality was determined after 24 hours through the “calcein/PI” assay. This evidence concerns the gene KCNH2 and neoplasm.