Thus, to determine whether hypoxic stress was influencing NDRG1 localisation in BC and its metastases, we cultured MDAMB231 (TNBC) and BT474 (HER2+) and their brain metastatic derivatives (.Br) in hypoxic conditions (3.8% O2) over a 24‐h time course. The gene discussed is NDRG1; the disease is breast cancer.