To investigate the impact of these immune cells on liver cancer, selected gene markers for cells of immune lineages including Kuffler cells (Cd68, Cd163, Lyz, C1qa, Aif1), T cells (Cd3d, Cd2, Il7r, Trbc2, Cd69, Cd4, Cd8, Cd11), B cells (Igkc, Jchain, Cd79a, Cd27, Cd74) and NK (Nkg7, Klrd1, Prf1, Cd7, Trdc) cells were analyzed for each spot. Here, CD3D is linked to liver cancer.