PCSK1 and pulmonary arterial hypertension: HGC27 and AGS cells were treated with PAH in combination with the ferroptosis inhibitor Ferrostatin-1 (Fer-1; 2 μmol/L), the apoptosis inhibitor Z-VAD-FMK (20 μmol/L), and the necroptosis inhibitor Necrostatin-1 (Nec-1; 20 μmol/L) (Figure 5A).