Therefore, we evaluated the adipocyte differentiation-associated genes like PPAR-γ, and CEBPα and adipokines like leptin, TNF-α, IL-6, etc. Leptin is an adipokine regulator of obesity acts as a metabolic regulator and shows a biphasic role in pre-adipocyte proliferation (39). This evidence concerns the gene IL6 and obesity due to melanocortin 4 receptor deficiency.