When HCC from DEN/HFD + EtOH-fed Dhcr7+/– and WT mice were compared, expression of YAP was suppressed in Dhcr7+/– mice, as shown by the reduced area of positive staining for YAP (↓3.0-fold), whereas expression of AFP and pospho-STAT3 was only slightly reduced in HCCs of DEN/ HFD + EtOH-fed Dhcr7+/– mice. This evidence concerns the gene AFP and hepatocellular carcinoma.