In turn, dsiRNA-based knockdown of DHCR7 reduced HepG2 proliferation (↓ PCNA, p <0.05; CCNE, p <0.05) and expression of MetALD-HCC markers (↓ AFP, p <0.05; GPC3, p <0.05; YAP, p <0.01). This evidence concerns the gene CCNE1 and hepatocellular carcinoma.