To explore the effect of targeted regulation of the MDK signaling pathway in GBM on the immune microenvironment within the tumor, we also used immunohistochemical staining technology to detect the expression characteristics of MDK, CXCL1, Ki67, CD3, CD4, CD8, CD68, iNOS, CD206, PD1, PD-L1, and Foxp3 molecules. This evidence concerns the gene CD8A and glioblastoma.