To explore how EGFRvIII mutant GBM regulates the expression of MDK, we performed transcriptome sequencing on EGFR WT and EGFRvIII mutant GBM cell lines, and conducted differentially expressed genes (DEGs) analysis on the two groups of samples (fold change >2 or <0.5, and p value < 0.05). The gene discussed is MDK; the disease is glioblastoma.