To further validate that EGFRvIII mutation contributes to the upregulation of MDK expression, we transfected wild-type (WT) EGFR and mutant EGFRvIII vectors into human GBM cell lines and evaluated the mRNA and protein expression levels of MDK in U87MG, U251MG, and T98MG cells by qPCR and Elisa. The gene discussed is MDK; the disease is glioblastoma.