To explore the effect of targeted regulation of the MDK signaling pathway in GBM on the immune microenvironment within the tumor, we also used immunohistochemical staining technology to detect the expression characteristics of MDK, CXCL1, Ki67, CD3, CD4, CD8, CD68, iNOS, CD206, PD1, PD-L1, and Foxp3 molecules. The gene discussed is CD4; the disease is neoplasm.