Then, we performed immunohistochemical staining on the tissue microarray using four markers: EGFRvIII, MDK, CD86, and CD206 (Fig. 2C), and selected representative immunohistochemical staining results for these four markers in EGFRvIII(−) and EGFRvIII(+) GBM patients from the tissue microarray (Fig. 2A). Here, CD86 is linked to glioblastoma.