When comparing KRAS mutant to wildtype, at 6h, oncolytic reovirus was effective at upregulating genes ATG5, BECN1, and MAP1LC3B, and at 24h, oncolytic reovirus was effective at upregulating all genes ATG5, BECN1, and MAP1LC3B, and ULK1. This has been found previously, where critical autophagy genes are upregulated post-reovirus treatment in mutant KRAS CRC [15]. This evidence concerns the gene ATG5 and colorectal carcinoma.