In this study, several tumor cell isolation techniques were compared for their efficiency in enriching tumor cells from MPEs: immunomagnetic enrichment of epithelial cells using EpCAM (MACS cell separation system, Miltenyi Biotec), negative selection via immunomagnetic CD45+ cell depletion (MojoSortTM Human CD45 Nanobeads, BioLegend), and size‐based separation of tumor cells utilizing cell strainers (pluriStrainer®). The gene discussed is PTPRC; the disease is neoplasm.