All four of the CpG sites found in the LMNA gene fragment expressed significant increases in methylation in PCOS patients compared to controls (P < 0.05); 12 out of 20 CpG sites in the LMNA promoter region had significantly higher methylation levels in PCOS (P < 0.05). No significant methylation differences were found in RPS4X and KCNJ11 genes, suggesting that LMNA hypermethylation is uniquely associated with PCOS-related IR. The gene discussed is KCNJ11; the disease is polycystic ovary syndrome.