Additionally, 13 genes were co-regulated by both miRNAs and hypomethylation, including BMP4, IRS1​​​, ETS1​​​​, FGFR1, CYP1B1, and KLF5. Specifically, genes involved in lipid metabolism, steroid synthesis, and glucose metabolism were regulated by abnormal DNA methylation, which may be implicated in the pathogenesis of PCOS [22]. The gene discussed is IRS1; the disease is polycystic ovary syndrome.