Abnormal DNA methylation at Pos.1 and Pos.4 of the AMHRII gene suggests enhanced protein expression and increased AMH levels; in PCOS patients, this methylation may alter gene activity by affecting transcription factor binding and DNA interactions; INSR gene methylation disrupts gene structure, leading to functional abnormalities and reduced receptor function; INSR dysfunction contributes to insulin resistance (IR) in PCOS; significant correlations were observed between gene methylation and IR. The gene discussed is AMH; the disease is polycystic ovary syndrome.