The study focused on three generations of family members with PCOS and IR, limiting its generalizability to other populations or individuals with different phenotypes of PCOS. The study was not longitudinal and did not depict methylation changes over time, which might correlate with PCOS progression or the effectiveness of potential interventions. In comparison to prior literature, some studies demonstrate a clear correlation between TGF-β1 expression and markers like testosterone levels or IR, while others find weak or no associations. This evidence concerns the gene TGFB1 and polycystic ovary syndrome.