Genome-wide methylation microarray screening was performed on peripheral blood mononuclear cells from three IR-PCOS patients and one healthy control to identify candidate genes associated with IR in PCOS, including LMNA, RPS4X, and KCNJ11. LMNA was selected for further validation, as RPS4X and KCNJ11 did not yield significant findings. Here, RPS4X is linked to polycystic ovary syndrome.