Genome-wide methylation-associated biomarkers were involved in the pathways of neurodegeneration-multiple diseases (13%), cancer-related pathways (DCC, RASSF1, WNT1, CTNNA2; 13%), and Hippo, calcium signalling, cell adhesion and immune system-related pathways (all 10%) (Figure 3D). Here, RASSF1 is linked to cancer.