The main pathways of methylome biomarkers were associated with calcium signalling (CACNA1E, RYR2, RYR3), cancer pathways (DCC, RASSF1, WNT1, CTNNA2), multiple neurodegenerative diseases (WNT1, DNAI1, RYR2, RYR3), immune system disorders and cell adhesion (HLA-DRA, HLA-DRB1, HLA-DRB5). Here, CACNA1E is linked to immune system disorder.