The differential expression of ATP citrate lyase (ACLY), acetyl-CoA carboxylase alpha (ACACA), fatty acid synthase (FASN), SCD, and SREBF1 was further validated via RT‒qPCR subsequent to magnetic-based cell sorting of co-cultured H2228 and H3122 tumor spheroids (Fig. S10). This evidence concerns the gene ACLY and neoplasm.