The HER2+EVs detected in the plasma of patients with documented 3+ HER2-expression in cancer tissue by histopathological analysis of biopsy material, however, formed a cluster in this scatter plot analysis, except for two patients (Fig. 4e), consistent with the high HER2 expression levels of the HER2+EVs we detected in these cancer patients (red dots). The gene discussed is ERBB2; the disease is cancer.