Interestingly, we found that the HER2+EVs from numerous patients who had no or low (2+/1+) HER2 expression (green dots) detected by histopathological investigation of cancer biopsies clustered in the same high probability zone for being HER2pos as the HER2+EVs from patients with 3+ HER2 expression in cancer tissue (Fig. 4e). This evidence concerns the gene ERBB2 and cancer.