Mechanistically, western blot analysis demonstrated that genetic disruption of NOXA (via either RNAi-mediated knockdown or CRISPR/Cas9 knockout) significantly attenuated RG7388-induced proteolytic cleavage of GSDME into its pore-forming N-terminal fragment in TP53mutant NSCLC cells (Fig. 4B-E). This evidence concerns the gene PMAIP1 and non-small cell lung carcinoma.