The number of efficient colonies in the N-Ras silencing ESCC cells were only 44–57% (Fig. 7C, blue and green) of that observed in the mock group (Fig. 7C, purple).To further assess the potential role of N-Ras in promoting esophageal cancer tumor metastasis, we used the transwell assay, which demonstrated that silencing N-Ras significantly impaired the metastatic potential of esophageal cancer cells (Fig. 7D, lanes 2–3). The gene discussed is NRAS; the disease is esophageal squamous cell carcinoma.