TUBA4A and cyst: Some of these proteins are housekeeping proteins often incorporated into EVs (e.g., HSP90B, TUBA1, GAPDH1) [43], but others are T. gondii specific proteins known to be secreted effectors (e.g., MAG1, GRA1, GRA2, MAG2, and GRA7) involved in cyst wall formation and host cell manipulation [5,53,54] (Fig 3E).