To elucidate the biological significance of VHL-mediated Vpx regulation in the context of viral infection, we generated PMA-differentiated Mono Mac 6 cells with shRNA-mediated VHL knockdown and evaluated their susceptibility to HIV-2 infection using a luciferase reporter virus system (HIV-2-Luc) (Fig 3A and 3B), as previously described [13]. This evidence concerns the gene VHL and viral infectious disease.