In order to identify possible functions of IFI27 within the innate immune responses against viral infections, IFI27 protein was overexpressed by transfecting a pCAGGS plasmid encoding for IFI27 fused to an HA tag (pCAGGS-IFI27-HA) in HEK-293T cells, or the cells were transfected with a pCAGGS plasmid encoding IFI44 fused to an HA tag (pCAGGS-IFI44-HA), or with an empty plasmid, as control, and these cells were subsequently transfected with poly(I:C), an analog of viral dsRNAs, in order to activate cellular pathways involved in the response to RNA viruses. Here, IFI44 is linked to viral infectious disease.