IFI27 and infection: In order to compare the effect of IFI27 overexpression in the context of viral infections, we transfected HEK-293T-hACE2 cells with either a pCAGGS-IFI27-HA plasmid or a pCAGGS empty plasmid, in combination with the pRL-RLuc plasmid, and 24 hpt, cells were infected with two different, unrelated, RNA viruses, during two different times post-infection, such SARS-CoV-2 (MOI 1), during 6 or 24 hours, or with VSV (MOI 0.1) during 8 h or 24 h (Fig 3A).