In line with the literature from peripheral endothelial cells (e.g., Kempe et al. 2005; Pan et al. 2016; Zhou et al. 2017), our transcriptomic analysis identified a consistent upregulation of the TNF (via NF‐κB) signaling pathway in the cerebral endothelial cells from an acute model of stroke and LPS stimulation from 15min to 24 h. The gene discussed is TNF; the disease is stroke disorder.