DAGLα is the enzyme responsible for synthesizing 2‐AG.[30] The absence of DAGLα gene expression in cells prevents the production and secretion of 2‐AG.[31] DAGLα knockout mice display varying degrees of anxiety and depressive tendencies.[32] Inhibiting DAGLα also impairs the extinction of fear responses in mice.[33] However, these studies used traditional pharmacological or gene knockout methods, lacking circuit‐ or synapse‐specificity, often leading to contradictory findings. This evidence concerns the gene DAGLA and Anxiety.