To test whether the inhibitory effect of the nanobody 1A5 on the proliferation of TKI‐resistant NSCLC cells is mediated by blocking the CMTM6‐EGFR interaction, we conducted depletion of CMTM6 to assess potential interference with the inhibition of NSCLC cell proliferation by 1A5. This evidence concerns the gene CMTM6 and non-small cell lung carcinoma.