Our RT‐qPCR results showed that TLR signaling‐related genes (tlr3, tlr4, tlr5, tlr7, tlr8a, tlr8b, tlr9, tab1, tbk1, nf‐κb, myd88, irf3, irf5, irf9), necroptosis signaling‐related genes (tlr3, tlr4, trif, ripk1, ripk3, caspase‐8, fadd, bax, bcl2), and inflammatory factors (tnf‐α, tnf‐β, il‐4, il‐6, il‐8, il‐10, il‐12, caspase‐1, cox‐2) were aberrantly expressed in zebrafish with IBD. Here, IRF3 is linked to inflammatory bowel disease.