Given the elevated expression of ABCG1 in renal cancer cell lines, including ACHN, A498, and 786-O (Figure 4A), We proceeded to construct ABCG1 knockout (hereinafter referred to as KO) cell lines and the corresponding control (hereinafter referred to as V2) cell lines to further explore the effects of ABCG1 deletion on the biological functions of ccRCC cells. The gene discussed is ABCG1; the disease is renal carcinoma.