The results demonstrated that, in comparison to the control group, the expression of Pip5k1c, Nlgn2, Fzd2, Cd86, Agpat1, and Degs2 showed a downward trend with an increase in the onset time of ischemic stroke, and the decrease was most significant in the MCAO_12 h group (p < 0.01). This evidence concerns the gene NLGN2 and ischemic stroke.