In summary, our current study systematically identified hyperacute-phase signature genes of AIS through bioinformatic screening, with subsequent validation focusing on the TLR2/TLR4/NF-κB pathway's temporal regulation of apoptosis and neuroinflammation via multi-timepoint in vivo and in vitro assays (Figure 10). The gene discussed is NFKB1; the disease is androgen insensitivity syndrome.