TNF and infection: In this study, the relative expressions of IL-10, MUC2, and ZO1 were increased, and the relative expressions of TNF-α and TGF-β1 were decreased in the groups treated with Pueraria extracts, which may be because the changes in the microbial community described above, by improving gut barrier function and reducing the risk of pathogen infection, indirectly affected the function of the immune system.