To further confirm that HSD3B7 was a culprit in the development of HCC induced by hepatocyte-specific knocking out of Mir122, a combination of c-Myc/sgTP53, with or without miR-122 and HSD3B7, was delivered into hepatocytes in C57BL/6J mice livers via HDI of the transposon vectors (Figures 6D and S5). The gene discussed is HSD3B7; the disease is hepatocellular carcinoma.