The expressions of five hub genes were negatively associated with BC lymph node metastasis, including NAB1 (P < 0.001), RFX5 (P = 0.011), S100B (P = 0.008), S1PR2 (P < 0.001) and VEGFA (P < 0.001) while F2R (P < 0.001) and IKZF2 (P = 0.004) showed positive association (Figures 6A–G). This evidence concerns the gene S1PR2 and breast cancer.