Then, we put 19 genes with P value <0.001 into multivariate Logistic regression analysis via the “Forward: LR” method and established a seven-gene immune-related signature for predicting BC lymph node metastasis, including F2R, IKZF2, NAB1, RFX5, S100B, S1PR2 and VEGFA. Here, NAB1 is linked to breast cancer.