Additionally, we used a KPC [C57BL/6-Krastm1(LSL-G12D)Trp53tm1(LSL-R172H)Tg(Pdx1-Cre)] transgenic mouse model, which spontaneously develops pancreatic cancer, and administered a combination of D166 and anti-PD-1. Here, PDCD1 is linked to familial pancreatic carcinoma.