To investigate the cytolytic activity of 8F4CAR-iNK T cells mediated by the 8F4CAR and the iNK-TCR, we co-cultured the effector iNK T cells with PR1/HLA-A2+ or αGalCer/CD1d+ leukemia cell lines (Figure 4A), and PR1/HLA-A2+ primary AML patient samples (Figure 4B), then assessed the specific killing of target cells. This evidence concerns the gene CD1D and leukemia.