Expressing IFNβ that inhibits the progression of melanoma by remodeling the TME. In comparison to VNP, VNP-IFNβ mobilized more macrophages and neutrophils with anti-tumor phenotypes in lung metastases and activated DCs differentiation, which stimulated the anti-tumor immune responses of CD4+ T cells, and finally the progression of melanoma inhibited. The gene discussed is CD4; the disease is neoplasm.