Our results revealed significantly upregulated PLCH1 mRNA expression in all breast cancer cell lines (MDA-MB-231, MDA-MB-468, BT-474, SKBR-3, and MCF-7) compared to normal breast epithelial cells MCF-10A (Figure 5E, P < 0.01 to P < 0.0001), with the highest expression observed in SKBR-3 and BT-474 cells. Here, PLCH1 is linked to breast cancer.