Firstly, the matrix gel utilized in organoid culture is primarily derived from the basement membrane matrix of Engelbreth-Holm-Swarm mouse sarcoma (Matrigel), which contains matrix proteins such as laminin, collagen IV, and nestin, along with various growth factors including TGF-β, epidermal growth factor, and insulin-like growth factor. This evidence concerns the gene TGFB1 and sarcoma.